| 2023-09-30 14:00:41September 30, 2023 |   Harlequins vs Trailfinders Women | 14:00:412:00 pm | Twickenham Stoop |
| 2023-10-14 15:00:55October 14, 2023 |  Trailfinders Women vs Gloucester-Hartpury | 15:00:553:00 pm | Trailfinders Sports Club |
| 2023-10-21 15:00:12October 21, 2023 |  Trailfinders Women vs Saracens | 15:00:123:00 pm | Trailfinders Sports Club |
| 2023-10-28 14:00:44October 28, 2023 |  Loughborough Lightning vs Trailfinders Women | 14:00:442:00 pm | Loughborough University |
| 2023-11-18 15:00:23November 18, 2023 | Trailfinders Women vs Harlequins | 15:00:233:00 pm | Trailfinders Sports Club |
| 2023-11-25 16:45:12November 25, 2023 | Trailfinders Women vs Saracens | 16:45:124:45 pm | Trailfinders Sports Club |
| 2023-12-09 15:00:33December 9, 2023 |  Trailfinders Women vs Leicester Tigers | 15:00:333:00 pm | Trailfinders Sports Club |
| 2023-12-16 13:00:09December 16, 2023 |  Bristol Bears vs Trailfinders Women | 13:00:091:00 pm | Shaftesbury Park |
| 2023-12-22 18:30:26December 22, 2023 |  Trailfinders Women vs Sale Sharks | 18:30:266:30 pm | Trailfinders Sports Club |
| 2024-01-07 12:30:15January 7, 2024 |  Exeter Chiefs vs Trailfinders Women | 12:30:1512:30 pm | Sandy Park |
| 2024-01-13 16:45:17January 13, 2024 | Trailfinders Women vs Gloucester-Hartpury | 16:45:174:45 pm | Trailfinders Sports Club |
| 2024-01-20 14:00:31January 20, 2024 | Loughborough Lightning vs Trailfinders Women | 14:00:312:00 pm | cinch Stadium at Franklin’s Gardens |
| 2024-02-03 14:30:47February 3, 2024 | Saracens vs Trailfinders Women | 14:30:472:30 pm | StoneX Stadium |
| 2024-02-23 19:00:47February 23, 2024 | Leicester Tigers vs Trailfinders Women | 19:00:477:00 pm | Mattioli Woods Welford Road |
| 2024-03-02 17:00:29March 2, 2024 | Trailfinders Women vs Bristol Bears | 17:00:295:00 pm | Trailfinders Sports Club |
| 2024-03-09 14:00:17March 9, 2024 | Sale Sharks vs Trailfinders Women | 14:00:172:00 pm | Salford Community Stadium |
| 2024-04-21 14:00:27April 21, 2024 | Trailfinders Women vs Leicester Tigers | 14:00:272:00 pm | Trailfinders Sports Club |
| 2024-04-28 14:00:29April 28, 2024 | Harlequins vs Trailfinders Women | 14:00:292:00 pm | Twickenham Stoop |
| 2024-05-11 15:00:18May 11, 2024 | Trailfinders Women vs Exeter Chiefs | 15:00:183:00 pm | Trailfinders Sports Club |
| 2024-05-19 14:00:15May 19, 2024 | Gloucester-Hartpury vs Trailfinders Women | 14:00:152:00 pm | Kingsholm |
| 2024-05-25 12:30:12May 25, 2024 | Trailfinders Women vs Loughborough Lightning | 12:30:1212:30 pm | Trailfinders Sports Club |
| 2024-06-02 15:00:45June 2, 2024 | Harlequins vs Trailfinders Women | 15:00:453:00 pm | Twickenham Stoop |
