| 2026-10-31 15:00:00October 31, 2026 |   Ealing Trailfinders vs Doncaster Knights | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2026-11-07 18:00:00November 7, 2026 |  Ampthill vs Ealing Trailfinders | 18:00:006:00 pm | Elior Champ Rugby | 2026-2027 | Dillingham Park |
| 2026-11-15 14:00:00November 15, 2026 |  Ealing Trailfinders vs Coventry | 14:00:002:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2026-11-21 13:00:00November 21, 2026 |  Caldy vs Ealing Trailfinders | 13:00:001:00 pm | Elior Champ Rugby | 2026-2027 | Paton Field |
| 2026-12-05 15:00:00December 5, 2026 |  Worcester Warriors vs Ealing Trailfinders | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Sixways Stadium |
| 2026-12-12 15:00:00December 12, 2026 |  Ealing Trailfinders vs Bedford Blues | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2026-12-19 15:00:00December 19, 2026 |  Rotherham Titans vs Ealing Trailfinders | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Clifton Lane |
| 2026-12-27 14:00:00December 27, 2026 |  Ealing Trailfinders vs Nottingham | 14:00:002:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2027-01-01 15:00:50January 1, 2027 | Nottingham vs Ealing Trailfinders | 15:00:503:00 pm | Elior Champ Rugby | 2026-2027 | Lady Bay Sports Ground |
| 2027-01-23 15:00:00January 23, 2027 | Ealing Trailfinders vs Worcester Warriors | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2027-01-30 15:00:06January 30, 2027 | Ealing Trailfinders vs Ampthill | 15:00:063:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2027-02-06 15:00:00February 6, 2027 | Doncaster Knights vs Ealing Trailfinders | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Castle Park |
| 2027-02-13 15:00:00February 13, 2027 |  Ealing Trailfinders vs Blackheath | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2027-02-20 15:00:00February 20, 2027 |  Hartpury vs Ealing Trailfinders | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Hartpury Stadium |
| 2027-02-27 15:00:00February 27, 2027 |  Ealing Trailfinders vs Cornish Pirates | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2027-03-20 15:00:00March 20, 2027 | Ealing Trailfinders vs Caldy | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2027-03-27 15:00:00March 27, 2027 | Coventry vs Ealing Trailfinders | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Butts Park Arena |
| 2027-04-03 15:00:00April 3, 2027 | Bedford Blues vs Ealing Trailfinders | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Goldington Road |
| 2027-04-10 15:00:00April 10, 2027 | Ealing Trailfinders vs Rotherham Titans | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
| 2027-04-17 15:00:00April 17, 2027 |  Richmond vs Ealing Trailfinders | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Richmond Athletic Ground |
| 2027-04-24 15:00:00April 24, 2027 |  Ealing Trailfinders vs Chinnor | 15:00:003:00 pm | Elior Champ Rugby | 2026-2027 | Trailfinders Sports Club |
