| 2025-11-01 15:00:03November 1, 2025 |   Ealing Trailfinders vs Hartpury RFC | 15:00:033:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2025-11-08 14:00:43November 8, 2025 |  Ampthill vs Ealing Trailfinders | 14:00:432:00 pm | Championship | 2025-2026 | Dillingham Park |
| 2025-11-16 15:00:27November 16, 2025 |  Ealing Trailfinders vs Doncaster Knights | 15:00:273:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2025-11-29 15:00:29November 29, 2025 |  London Scottish vs Ealing Trailfinders | 15:00:293:00 pm | Championship | 2025-2026 | Richmond Athletic Ground |
| 2025-12-06 15:00:57December 6, 2025 |  Ealing Trailfinders vs Caldy | 15:00:573:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2025-12-12 19:45:36December 12, 2025 |  Nottingham vs Ealing Trailfinders | 19:45:367:45 pm | Championship | 2025-2026 | The Bay |
| 2025-12-20 15:00:10December 20, 2025 |  Ealing Trailfinders vs Cambridge | 15:00:103:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2025-12-27 14:30:22December 27, 2025 |  Cornish Pirates vs Ealing Trailfinders | 14:30:222:30 pm | Championship | 2025-2026 | Mennaye Field |
| 2026-01-03 15:00:26January 3, 2026 |  Ealing Trailfinders vs Worcester Warriors | 15:00:263:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2026-01-23 19:45:17January 23, 2026 |  Chinnor vs Ealing Trailfinders | 19:45:177:45 pm | Championship | 2025-2026 | Chinnor RFC |
| 2026-01-31 15:00:42January 31, 2026 |  Ealing Trailfinders vs Richmond | 15:00:423:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2026-02-07 15:00:33February 7, 2026 |  Ealing Trailfinders vs Coventry | 15:00:333:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2026-02-14 15:00:56February 14, 2026 | Hartpury RFC vs Ealing Trailfinders | 15:00:563:00 pm | Championship | 2025-2026 | Hartpury University |
| 2026-02-21 15:00:19February 21, 2026 | Ealing Trailfinders vs Ampthill | 15:00:193:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2026-02-28 14:30:33February 28, 2026 | Doncaster Knights vs Ealing Trailfinders | 14:30:332:30 pm | Championship | 2025-2026 | Castle Park |
| 2026-03-21 15:00:49March 21, 2026 | Ealing Trailfinders vs London Scottish | 15:00:493:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2026-03-28 15:00:01March 28, 2026 | Caldy vs Ealing Trailfinders | 15:00:013:00 pm | Championship | 2025-2026 | Paton Field |
| 2026-04-04 15:00:30April 4, 2026 | Ealing Trailfinders vs Nottingham | 15:00:303:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2026-04-11 15:00:04April 11, 2026 | Cambridge vs Ealing Trailfinders | 15:00:043:00 pm | Championship | 2025-2026 | Ellgia Fields |
| 2026-04-18 15:00:14April 18, 2026 | Ealing Trailfinders vs Cornish Pirates | 15:00:143:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
| 2026-05-02 15:00:12May 2, 2026 | Worcester Warriors vs Ealing Trailfinders | 15:00:123:00 pm | Championship | 2025-2026 | Sixways Stadium |
| 2026-05-09 15:00:59May 9, 2026 |  Ealing Trailfinders vs Bedford Blues | 15:00:593:00 pm | Championship | 2025-2026 | Trailfinders Sports Club |
